Developed by Haruo Nakano, Tokyo university of the pharmacy and life sciences in 2019.The HA-tag was introduced into zygotes by CRISPR/Cas9 genome editing. Jcl:ICR (CLEA Japan Inc.)The ATF5-HA knock-in male G0 offspring was mated with wild type females, and then propageted by sibling crossing (Heterozygous x Heterozygous or Homozygous x Homozygous). Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Tissue Res. 2021 Apr 7. doi: 10.1007/s00441-021-03450-7.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. C (3-6 months) C（3〜6か月） ATF5-Hemagglutinin (HA) knock-in mice. A HA-tag sequence was integrated into the stop codon site of ATF5 gene at an in-flame position. ATF5-Hemagglutinin (HA) knock-in mice. A HA-tag sequence was integrated into the stop codon site of ATF5 gene at an in-flame position. true 条件を付加する。利用者は提供承諾書を用いて、事前に寄託者の承諾を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Cell Tissue Res. 2021 Apr 7. doi: 10.1007/s00441-021-03450-7.<br>研究成果の公表にあたって謝辞の表明を必要とする。 Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: CRISPR/Cas9 genome edited bioresources (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/Broad_MTA_J.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/Broad_MTA_E.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> RBRC11378 ATF5-HA knock-in, ICR-ATF5HA KI ATF5-HA knock-in, ICR-ATF5HA KI <a href='https://brc.riken.jp/mus/pcr11378'>Genotyping protocol -PCR-</a> influenza virus HA (hemagglutinin) tag sequence Developed by Haruo Nakano, Tokyo university of the pharmacy and life sciences in 2019.The HA-tag was introduced into zygotes by CRISPR/Cas9 genome editing. Jcl:ICR (CLEA Japan Inc.)The ATF5-HA knock-in male G0 offspring was mated with wild type females, and then propageted by sibling crossing (Heterozygous x Heterozygous or Homozygous x Homozygous). ICR-Atf5<em2(Atf5/HA)Yuta>