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<Asymmetric unit>
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PDB ID1sud  sequence information (FASTA format)   
DescriptorSUBTILISIN BPN' CRB-S3 (E.C.3.4.21.62) MUTANT WITH MET 50 REPLACED BY PHE, TYR 217 REPLACED BY LYS, ASN 218 REPLACED BY SER, AND SER 221 REPLACED BY CYS (M50P,Y217K, N218S,S221C)
TitleCALCIUM-INDEPENDENT SUBTILISIN BY DESIGN
Functional KeywordsHYDROLASE(SERINE PROTEINASE)
Biological sourceBacillus amyloliquefaciens
Cellular location [UNP - P00782] Secreted
Total number of polymer chains1
Total molecular weight27733.1 (the details in Structural Details Page)
AuthorsGallagher, T. , Bryan, P. , Gilliland, G.L. (deposition date : 1992-06-10, release date : 1994-01-31)
Primary citationGallagher, T. , Bryan, P. , Gilliland, G.L.
Calcium-independent subtilisin by design.
Proteins, 16:205 - 213, 1993.(PubMed : 8332608)  (DOI: 10.1002/prot.340160207)
Experimental methodX-RAY DIFFRACTION ( 1.9[Å] )
Other Database Information
Yorodumi , CATH , CE , FSSP , SCOP , VAST , UniProt ( P00782 ) , eF-site , KEGG ( EC 3.4.21.62 ) , PISA